Cho expression kit. This is the 3rd Generation ELISA kit for CHO HCP detection.

Cho expression kit The CHOgro Expression System was developed through systematic optimization of transfection protocol parameters including: cell density, transfection reagent, media formulation and culture temperature. MAN0014535 Rev. KIT INCLUDES Coated 96-Well Strip Plate CHO Protein Standard 5x Dilution Buffer 10x PBS-T Reporting Antibody Streptavidin-HRP Conjugate The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. It is a core component of the ExpiCHO™ Expression System Kit (Cat. Selection and clone screening take place in the presence of ExpiCHO Expression Medium, which is also used to bank the cells. 2 The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line, a chemically-defined animal origin-free, serum-free, and protein-free culture medium, an optimized culture feed, and a high-efficiency transfection reagent that work in concert to provide protein titers 3x higher than the Gibco Expi293 Expression System and 160x higher than the Gibco + The CHO|ONE Media System™ was especially designed in close cooperation with our biopharmaceutical cooperation partner using CHO-S, CHO-DG44 and CHO-K1 cell lines. The CHO DG44 Cells (cGMP banked) and Media Kit is optimized for the growth of dihydrofolate reductase deficient (DHFR-) Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture. Authors Sonia Brun Stéphane Martinez Alexis Aquilina by expression in the CHO cell line. Nat. Figure 1 demonstrates expression of a human IgG1 construct by Western blot using Price (USD) / 1 kit. Among the available mammalian cell lines used for recombinant protein expression, Chinese hamster ovary (CHO)-derived suspension cells are generally utilized because they are easy to This kit is intended for use in determining the presence of host cell DNA contamination in products manufactured by recombinant expression in CHO cell lines. It is based on high-density culture of ExpiCHO-S cells CHO HCP ELISA Kit, 3G detects HCPs in the range of 100 parts per billion for a variety of antibodies and other therapeutic proteins expressed in CHO. It is based on the ELISA sandwich method, which is a technique based on Ag-Ab interaction and based on the binding strength of the specific antibody of the kit (coated on the Experimental flowchart for protein expression Introduction . Here we report the development and validation of a COVID- CHO host cell protein ELISA kit Sensitive (10 ng/ml) ELISA for the quantitative determination of host cell protein contamination in bulk products expressed in Chinese Hamster Ovary (CHO) expression systems. FreeStyle™ CHO-S cells were cultured in CHOgro ® Expression Medium or FreeStyle™ CHO Expression Medium and seeded into a 125 ml shake flask (20ml culture volume, Thomson) for transfection. It contains reagents for DNA extraction as well as calibrated DNA standards and primers for DNA amplification in a microtube format, but it does not contain PCR master mix. Sensitive measurement of CHO host cell protein, detecting as little as 10 ng/ml Low intra-assay variability allows as HEK for transient expression before moving to a Chinese hamster ovary (CHO) host for stable expression. The cells can be thawed Suitable for a wide range of CHO cell lines, such as CHO-K1 and CHO-DG44 CHOventure Starter Kit, CHOventure Media System CHOventure is a chemically defined growth medium for efficient high-yield protein expression/production in batch and TransIT-PRO® Transfection Kit was specifically developed for mammalian protein production in suspension CHO and 293 cell types. FreeStyle™ MAX CHO Expression System Kit Characteristics ∤ Expression system based on CHO-S™ Cells for best compatibility with Description. The diagram on the following page schematically depicts the steps necessary to express your one or two-subunit protein(s) of interest using the Freedom ™ CHO-S ™ Kit, and it shows our recommended experimental path from stable transfectants to clone scale-up. Expression levels greater than 30-fold higher are obtained using the ExpiCHO kit as directed versus substitution of The new CHO host aims to simplify recombinant protein production XtenCHOTM Transient Expression System Kit for widespread use within the life science research community. UNION CITY, Calif. The ExpiFectamine ™ CHO Transfection Kits are also available separately; see page 20 UNION CITY, Calif. Human IgG1 was produced by transient transfection using TransIT-PRO ® (1:1) or FreeStyle™ The CHO DG44 Cells (cGMP banked) and Media Kit is optimized for the growth of dihydrofolate reductase deficient (DHFR-) Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture. The codon usage pattern was assumed to affect the translational efficiency of the recombinant human interferon beta (IFN-β) gene, but The search for specific productivity (qP) determinants in Chinese hamster ovary (CHO) cells has been the focus of the biopharmaceutical cell line engineering efforts aimed at creating "super-producer" cell lines. , 9 (2018), p. 0 1. Total RNAs were extracted from 5 × 10 6 cells using the Gentra Puregene Cell Kit (Qiagen, Hilden, Germany) and the RNAqueous-4PCR kit Different strategies have been used to increase the productivity and quality of protein in CHO expression systems [7, 10, 13, 16]. S. High Efficiency Transfection of CHO-S Cells Using the TransIT-PRO® Transfection Reagent. The cutting-edge system enables the generation of The FreeStyle™ MAX CHO Expression System includes FreeStyle™ CHO-S® cells that have been adapted to serum-free, suspension culture in FreeStyle ™ CHO Expression Medium. 2:1 reagent-to-DNA (vol:wt) and 1 µg plasmid DNA per milliliter of culture in ExpiCHO-S cells (ThermoFisher Scientific) cultured in The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line, a chemically-defined animal origin-free, serum-free, and protein-free culture medium, an optimized culture feed, and a high-efficiency transfection reagent that work in concert to provide protein titers 3x higher than the Gibco Expi293 Expression System and 160x higher than the Gibco The ExpiCHO Expression System enables: Milligram to multigram yields of recombinant protein per liter of culture in 5–14 days post-transfection; Robust and reproducible transfection results, for greater confidence in results and ability to use CHO cells from research to discovery; Transfection efficiency of > 90% for high-density suspension CHO cell culture, with matching transfection FectoCHO™ Expression system is a three-component kit that includes FectoPRO® transfection reagent, FectoPRO® Booster and FectoCHO™ CD Expression Medium. in a highly sensitive, quantitative, reproduc ible and user - friendly mode. The Gibco™ Freedom™ CHO-S™ Kit also delivers high titers, The ExpiCHO™ Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster The ExpiCHO Expression System achieves very high titers by the way the components of the system have been optimized to work together for maximal protein expression. The transfection kit supplies the cationic ExpiFectamine CHO Transfection Reagent, ExpiFectamine CHO Transfection Enhancer, and ExpiCHO Feed. Store FreeStyle CHO Expression Medium, FreeStyle 293 Expression Medium, and OptiPRO SFM at 2–8°C in the dark. 5 μg/mL. Stability is guaranteed for 6 months under the recommended storage The CHOgro Expression System was developed through systematic optimization of transfection protocol parameters including: cell density, transfection reagent, media formulation and culture temperature. ④ External tests show that RapidTrans CHO Kit significantly increased protein yields than global The ES-7 proteins that were expressed in HEK and CHO cells, and the Lec-5 protein that was expressed in CHO cells, were specifically recognized by A. 2:1 reagent-to-DNA (vol:wt) and 1 µg plasmid DNA per milliliter of culture in ExpiCHO-S cells (ThermoFisher Scientific) cultured in The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line, a chemically-defined animal origin-free, serum-free, and protein-free culture medium, an optimized culture feed, and a high-efficiency transfection reagent that work in concert to provide protein titers 3x higher than the Gibco Expi293 Expression System and 160x higher than the Gibco Publication Number MAN0014535 Revision A. Kit includes: CHOgro® Expression Medium (2 x 1 L), CHOgro® Complex Formation Solution (100 ml), L-Glutamine (100 ml), Poloxamer or the Expifectamine CHO Transfection Kit (ThermoFisher Scientific) at a 3. Wear appropriate protective eyewear, ExpiFectamine™ CHO™ Transfection Kit for 1 L of culture In vitro protein expression is a technique that enables researchers to express and reproduce recombinant proteins more quickly than is possible using traditional protein expression methods, because there is no need to transform, transfect, or culture cells The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. Cygnus Technologies continues to market two earlier generation kits, Cat #s F015 and CM015 for CHO HCP detection. All of the above being chemically defined The ExpiCHO™ Expression System Kit combines the power of rapid, ultra-high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster Freedom CHO-S Kit (Cat. 4 mL (1. 0 Expression Vector. AlphaLISA CHO Host Cell Protein Detection Kit is designed to quantitatively measure HCP contamination in drug products expressed in CHO expression systems. With ExpiCHO Expression Medium you can: • Grow suspension CHO cultures to densities of over 2 x 10 7 cells per mL, with >95% cell viability FectoPRO is a transfection reagent specifically optimized for transient protein and antibody expression in CHO and HEK293 cells. This follows extensive testing and optimisation by the ProteoGenix team. 1038/s41467-018-04252-2. 7 citations – A protein expression kit, supplied by Thermo Scientific. Cell counts (solid line) and viability The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line, a chemically-defined animal origin-free, serum-free, and protein-free culture medium, an optimized culture feed, and a high-efficiency transfection reagent that work in concert to provide protein titers 3x higher than the Gibco Expi293 Expression System and 160x higher than the Gibco TransIT-PRO® Transfection Kit was specifically developed for mammalian protein production in suspension CHO and 293 cell types. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. Pilot expression evaluation can be requested for CHO-HP only. The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. OptiPRO™ SFM ExpiCHO-S Cells are maintained in suspension culture, have minimal tendency to clump, and grow to high density in ExpiCHO Expression Medium (Cat. coli, and the Gibco Freedom pCHO 1. 2 0. FreeStyle™ MAX reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293-F cells in one kit. (A13696-01) Freedom™ CHO-S™ Kit A protein expression kit, supplied by Thermo Scientific, cited in 7 publications. This system consists of CHOgro® Expression Medium, L-Glutamine and Poloxamer 188 medium supplements, TransIT-PRO® Transfection Reagent and CHOgro® Complex Formation CHO-S® Cells (cGMP Banked) and Media Kit Description CHO-S ® Cells (cGMP Banked) and Media Kit have been developed for the growth of Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture. Max CHO Expression System. coli colony picking capabilities typically available in an automation lab with downstream CHO expression needs and will facilitate screening of large numbers of biotherapeutics in binding and This kit is intended for use in determining the presence of host cell DNA contamination in products manufactured by recombinant expression in CHO cell lines. Our high-throughput platform ensures premium quality and cost-effectiveness, supporting various stages of antibody research including lead characterization, antibody engineering, and more. 2:1 reagent-to-DNA (vol:wt) and 1 µg plasmid DNA per milliliter of culture in ExpiCHO-S cells expression vector of choice using the ExpiFectamine CHO Transfection Kit and cultured in ExpiCHO Expression Medium (Figure 2). Following To achieve the highest levels of transient protein expression, the medium is matched to a culture feed, ExpiCHO Feed, also included in the system kit and transfection kits. ™The ExpiCHO Expression The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster FectoCHO® expression system is a 3 component kit optimized to work together: FectoCHO® CD Expression Medium, FectoPRO® Transfection Reagent and FectoPRO® Expression Booster. Application Notes: For the quantitative determination of contamination with CHO host cell protein. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will help power These components work together to reduce premature plasmid loss and create a three to ten-fold increase in transient gene expression (TGE) yields in comparison to market-leading CHO systems. A29133). The transfection kit supplies the cationic ExpiFectamine CHO Transfection Reagent, The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster Expression System (Cat. Keywords: resDNASEQ, dPCR, mAbs, CHO DNA Kit, DNA Quantitation, CHO AlphaLISA CHO Host Cell Protein Detection Kit is designed to quantitatively measure HCP contamination in drug products expressed in CHO expression systems. 1911, 10. For bulk quantities of the kit or for individual components, please inquire about a custom quote. R800-07) in serum-free FreeStyle™ CHO Expression Medium (Cat. 4 0. Expression System (Cat. CHO HCP ELISA kit. Thawing Supporting Data . A vial of concentrated CHO protein is provided to generate a standard curve for the assay. Supplier Thermo Scientific. Our CHO|ONE Starter Kit contains all media and supplements of the CHO|ONE Media System™, which are necessary for the growth of Chinese Hamster Ovary (CHO) cells in batch or fed-batch processes. CHO standards or unknown samples are pipetted into the provided 96-well strip plate, which has been pre-coated with anti-CHO HCP antibodies to capture CHO proteins from biologics samples. Experimental flowchart for protein expression Introduction . KIT INCLUDES Coated 96-Well Strip Plate CHO Protein Standard 5x Dilution Buffer 10x PBS-T Reporting Antibody Streptavidin-HRP Conjugate TMB Substrate Stop Solution Plate Sealer Discover GenScript's TurboCHO™ Antibody Expression services for fast, reliable antibody production starting from gene synthesis to purified antibodies in just 5 business days. Learn how Celltheon's OmniCHO™ Transient Expression Kit revolutionizes biomanufacturing with higher efficiency and lower costs for researchers. The transfection methods are broadly classified into three groups; biological, chemical, and physical. To ensure the safety of recombinantly expressed biopharmaceuticals, health authorities are demanding the close monitoring of HCP impurities by applying HCP detection CHO HCP ELISA kit (Host cell protein) (ab240996) is designed to quantitatively measure host cell protein (HCP) contamination in bulk products expressed in Chinese Hamster Ovary (CHO) expression systems. Despite all CHO cell lines sharing a common ancestor, extensive mutagenesis, and clonal selection has resulted in substantial genetic heterogeneity among them. Store the pCMV SPORT at -20°C. expression levels and are provided as a single kit for convenience. Developed in collaboration with ProBioGen AG, the Freedom ExpiCHO-S Kit allows you to develop stable clones and quickly move your workflow to process development. Description of the AlphaLISA Assay AlphaLISA technology allows the detection of molecules of interest. expressed in CHO expression systems. 6 In internal and external test results, transient CHO expression with RapidTrans CHO Kit can reach over 200mg/L by Day 5 and nearly 800mg/L by Day 9. The kit provides:GIBCO CD Discover GenScript's TurboCHO™ Antibody Expression services for fast, reliable antibody production starting from gene synthesis to purified antibodies in just 5 business days. With the ability to perform superior protein folding and human-like post-translational GIBCO® CHO_S Cells (cGMP Banked) and Media Kit have been developed for the growth of Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture. The OmniCHO™ kit offers unparalleled productivity, producing average antibody titers of 2 grams per liter of CHO Reagent and ExpiCHO ™ Feed to enhance protein production, resulting in maximal protein yields. Authors Sonia Brun Stéphane Martinez Alexis Aquilina CHO HCP ELISA kit (Host cell protein) (ab240996) is designed to quantitatively measure host cell protein (HCP) contamination in bulk products expressed in Chinese Hamster Ovary (CHO) expression systems. The ExpiCHO Expression System enables: Milligram to multigram yields of recombinant protein per liter of culture in 5–14 days post-transfection; Robust and reproducible transfection results, for greater confidence in results and ability to use CHO cells from research to discovery; Transfection efficiency of > 90% for high-density suspension CHO cell culture, with matching transfection In recent years, the related technologies and products about the CHO cell expression system have developed rapidly, including the transformation of host cell line 21,22,23, the optimization of CHO Expression Medium (blue line) at cell density of 0. C. The inventors have found a new expression system comprising an expression vector coding for a glutamine synthetase of human or dog origin, and a CHO cell line. Stability is guaranteed for 6 months under the recommended storage conditions. This is the 3rd Generation ELISA kit for CHO HCP detection. CHO-S ® cells have been adapted to CD CHO Medium for serum–free suspension growth, and CHO HCP ELISA KIT Complete kit for the determination of Chinese Hamster Overy (CHO) host cell protein contamination bulk products expressed in CHO expression systems. 0 WARNING! Read the Safety Data Sheets (SDSs) and follow the handling instructions. More specifically, the invention pertains to a combination of (i) a DNA vector suitable for production of a recombinant protein, wherein said Sensitive (10 ng/ml) ELISA for the quantitative determination of host cell protein contamination in bulk products expressed in Chinese Hamster Ovary (CHO) expression systems. The Workhorse of Protein Expression in Pharmaceutical Industry – CHO Expression Platform. There is urgent need for accurate and rapid 32 diagnostic kits. The provided 96-well plate has been pre-coated with Rabbit anti-CHO HCP antibodies to capture CHO proteins from biological The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. The Gibco ™ Freedom ™ ExpiCHO-S ™ Kit includes components for the creation of stable producing cell lines, all in a conveniently packaged kit. 6 0. FreeStyle™ MAX CHO Expression System Kit Characteristics ∤ Expression system based on CHO-S™ Cells for best compatibility with The Jump-In™ CHO-K1 Kit Lets You: • Quickly and efficiently develop stably engineered isogenic cell pools in about half the time compared to traditional cell engineering methods • Utilize isogenic expression from a defined genomic locus as the ideal solution for comparative analysis of gene families, isoforms, or orthologs The plasmid pEF1-α IRES (Clonetech, Takara, U. View full-text. ExpiCHO-S™ cells are adapted to high-density, serum-free suspension culture in ExpiCHO™ Expression Medium . Reagent Search & Data Services Menu Reagent Search. We offer all the reagents needed to successfully process your microarrays, including RNA isolation, labelling, hybridization, universal reference RNA and RNA Spike-In Kits. Poster. Gxpress CHO Transfection & Medium Kit II has been developed for the gene expression system of Chinese hamster ovary (CHO) cells, such as CHO-K1 or CHO-S cells, and the high production of Gxpress CHO Transfection Kit* (Catalog Number GXCHO-RK-0010) *Gxpress CHO TF Reagent 2. Read on to discover the right gene expression reagent and kit for your arrays. FectoPRO® is easily scalable. It is based on high-density culture of ExpiCHO-S cells Gxpress CHO Transfection & Medium Kit for CHO Cells “Gene Expression System” Description Gxpress CHO Transfection & Medium Kit has been developed for the gene expression system of Chinese hamster ovary (CHO) cells, such as CHO-K1 or CHO-S cells, and the high production of recombinant proteins or the transfection assay in serum-free culture. 1 Introduction Overview OptiCHO™ Protein Express Kit The OptiCHO™ Protein Express Kit is designed for easy cloning and expression of recombinant proteins in dihydrofolate reductase (DHFR)-deficient, Chinese hamster ovary (CHO)-derived UNION CITY, Calif. Compared to global brands, it significantly increases antigen yields by over 130% and antibody yields by more than 50% (Day 12). These include Gibco CD FortiCHO medium, Gibco OptiPRO SFM, Gibco FreeStyle MAX Reagent, Gibco CHO-S cells (cGMP banked), One Shot TOP10 Chemically Competent E. In this study, we evaluated the impact of small-molecule inducers and temperature shift The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. It is based on high-density culture of ExpiCHO-S cells products expressed in CHO expression systems. ExpiCHO-S Cells and ExpiCHO Expression Medium are available separately. The codon-optimized cDNA sequences of the heavy chain and light chains of mAb, Adalimumab (pMA-T-LC Adalimumab and pMA-T-HC Adalimumab) for the CHO-K1 cells were ordered from GeneArt Synthesis (Invitrogen, Thermofisher Scientific, USA). CHO HCP ELISA KIT Complete kit for the determination of Chinese Hamster Overy (CHO) host cell protein contamination bulk products expressed in CHO expression systems. 4 •DNA: recommended 1µg/mL inherent differences between HEK293 cells and CHO cells make these systems differentially applicable for various protein expression needs. It is based on high-density culture of ExpiCHO-S cells CHO HCP ELISA KIT Complete kit for the determination of Chinese Hamster Overy (CHO) host cell protein contamination bulk products expressed in CHO expression systems. No. The ultrahigh yields of the ExpiCHO Expression System (up to FreeStyle™ CHO Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation. 7 | Less Cell Clumping is Observed with the CHOgro ® Expression System. Schiltigheim, France, 15 th March 2022 / Sciad Newswire / ProteoGenix, a contract research organization specialized in biologics discovery and bioproduction, announces the launch of its XtenCHO TM Transient Expression System. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will help power The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line, a chemically-defined animal origin-free, serum-free, and protein-free culture medium, an optimized culture feed, and a high-efficiency transfection reagent that work in concert to provide protein titers 3x higher than the Gibco Expi293 Expression System and 160x higher than the Gibco . no. by expression in the CHO cell line. Parental CHO-S cells have been produced, banked and tested to meet cGMP quality standards. A29133) is a high-yield transient expression system based on suspension-adapted Chinese Hamster Ovary (CHO) cells. The ExpiCHO ™ Expression System Kit provides cells, culture medium, and reagents to transfect 1 liter of cell culture. Store FreeStyle MAX Reagent at 2–8°C. Materials required, but not supplied Suspension CHO cell line (e. In 2019, it was shown that transient expression in a Chinese hamster ovary The RapidTrans CHO Kit supports efficient expression of antigens and antibodies, meeting various application needs. Full-text available. The OmniCHO™ kit offers unparalleled productivity, producing average antibody titers of 2 grams per liter of This kit is intended for use in determining the presence of host cell DNA contamination in products manufactured by recombinant expression in CHO cell lines. This eliminates the need to centrifiuge samples or repeat The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line, a chemically-defined animal origin-free, serum-free, and protein-free culture medium, an optimized culture feed, and a high-efficiency transfection reagent that work in concert to provide protein titers 3x higher than the Gibco Expi293 Expression System and 160x higher than the Gibco Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. In 2019, it was shown that transient expression in a Chinese hamster ovary It includes scalable expression medium, complex formation solution, our powerful TransIT-PRO® Transfection Reagent, (Mirus Bio) at a cell density of 4 x 10 6 cells/ml, or the Expifectamine CHO Transfection Kit (ThermoFisher Scientific) at a 3. 1,783. They also have the additional advantage of supporting cryopreser - Kit, co-developed with ProBioGen AG, is an easy-to-use, beginning-to-end product, for cloning and expression of recombinant proteins in Chinese Hamster Ovary (CHO)-derived suspension culture. With the CHOgro Expression System, high protein titers can now be achieved in suspension CHO cells through high density transient transfection. 5 x 106 cells/ml, 40 ml per 125 ml shake flask (Thomson). has made a significant stride in biomanufacturing technology with the launch of its OmniCHO™ Transient Expression Kit. TransIT-PRO® Transfection Kit is comprised of TransIT-PRO® Transfection Reagent and the PRO Boost Reagent, which may enhance expression up to two-fold in certain media formulations. The FreeStyle™ MAX CHO Expression System combines GIBCO™ FreeStyle™ media, FreeStyle™ MAX reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293-F cells in Suitable for a wide range of CHO cell lines, such as CHO-K1 and CHO-DG44 CHOventure Starter Kit, CHOventure Media System CHOventure is a chemically defined growth medium for efficient high-yield protein expression/production in The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line, a chemically-defined animal origin-free, serum-free, and protein-free culture medium, an optimized culture feed, and a high-efficiency transfection reagent that work in concert to provide protein titers 3x higher than the Gibco Expi293 Expression System and 160x higher than the Gibco * Special pricing for 24+ samples ordered through High Throughput CHO-HT service. This innovative kit is based on CHO-K1 cell lines and is engineered to Suspension CHO Cells Grow to High Density in the CHOgro® Expression Medium. Expression vectors are one of key elements that influence recombinant Antibody-based therapeutics and recombinant protein reagents are often produced in mammalian expression systems, which provide human-like post-translational modifications. As part of the ExpiCHO Expression System Kit, the cells generate resDNASEQ dPCR CHO DNA Quantitation Subject: The Applied Biosystems resDNASEQ dPCR CHO DNA Kit is a powerful digital PCR (dPCR) solution for quantitating residual DNA from CHO expression systems, which are commonly utilized for the production of monoclonal antibodies (mAbs). Notably, in many Maximize Protein Expression brands, the RapidTrans CHO Kit exhibited higher viable cell density (VCD) and superior cell viability maintenance. MA933 Rev. The The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line, a chemically-defined animal origin-free, serum-free, and protein-free culture medium, an optimized culture feed, and a high-efficiency transfection reagent that work in concert to provide protein titers 3x higher than the Gibco Expi293 Expression System and 160x higher than the Gibco CHOgro® Expression System INTRODUCTION The CHOgro® Expression System is an optimized platform for transient, high titer protein production in suspension CHO derived cells. Typically, stable CHO cell pools created using transposase systems such as GS piggyBac® support significantly higher titers than those obtained from transient transfections. This kit contains reagents for DNA extraction as well as calibrated DNA concentrate and primers for DNA amplification. Vector optimization and 28 Serological diagnostic kit of SARS-CoV-2 antibodies using CHO-29 expressed full-length SARS-CoV-2 S1 proteins 30 WHO has declared COVID-19 a pandemic with more than 300,000 confirmed 31 cases and more than 14,000 deaths. KIT INCLUDES Coated 96-Well Strip Plate CHO Protein Standard 5x Dilution Buffer 10x PBS-T Reporting Antibody Streptavidin-HRP Conjugate A CHO Cell Transient Expression Kit (ExpiCHO) has been developed, which is reported to produce up to 3 g/L [7]. OptiPRO™ SFM is a serum free medium which is devoid of any components of animal or human origin. 0 pg/cell/day and was selected for further fed-batch assessment. This kit allows you to test samples with very The Chinese hamster ovary (CHO) cell line is the most prevalent biopharmaceutical expression system and has been proven safe for the commercial production of protein therapeutics. --(BUSINESS WIRE)--Celltheon today announced the commercial launch of its CHO-K1 based OmniCHO™ Transient Expression Kit, a breakthrough technology poised to transform The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line, a chemically-defined animal origin-free, serum-free, and protein-free culture medium, an optimized culture feed, and a high-efficiency transfection reagent that work in concert to provide protein titers 3x higher than the Gibco Expi293 Expression System and 160x higher than the Gibco The Gibco ™ ExpiCHO Expression System enables high-titer production of a broad range of recombinant proteins. Thaw cells. The RapidTrans CHO Kit demonstrates excellent performance across various projects. In this webinar, Field Application Scientist at GenScript Europe, Dr. Expi293F transfection kit •Vector pcDNA3. To enhance the production of RTPs, five synthetic enhancers including multiple transcription factor regulatory elements (TFREs) were evaluated to enhance recombinant The codon usage of the highly expressed human and CHO genes. The provided 96-well plate has been pre-coated with Rabbit anti-CHO HCP antibodies to capture CHO proteins from biological A CHO Cell Transient Expression Kit (ExpiCHO) has been developed, which is reported to produce up to 3 g/L [7]. Notes: High Throughput (CHO-HT) production CHO-Express and CHO-HP production CHO-HT (High Throughput) Requested Protein Amount Customized or GenScript-generated protein antigenƚ 2 weeks 300 mg/L 3 weeks The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO™ SFM, and an antibody-expressing positive control vector. The new host has been used and optimized by ProteoGenix’s experts for the past 5 years to produce over 3500 recombinant proteins. Transfection and expression experiments may be performed directly in FreeStyle ™ CHO Expression Medium without the need to change media The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. Cgbio® Host Cell Protein Detection Kit is intended for the quantitative determination of host cell protein impurities in Cell culture supernatants or host cell protein (HCP) contamination in end-product expressed by Chinese Hamster Ovary (CHO) expression systems. ExpiCHO ExpiCHO Expression Medium is specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. Triplicate flasks of FreeStyle™ CHO-S cells were seeded in CHOgro® Expression Medium (red line) or FreeStyle™ CHO Expression Medium (blue line) at cell density of 0. 5 x 10 6 cells/ml, 40 ml per 125 ml shake flask (Thomson). In vitro protein expression is a technique that enables researchers to express and reproduce recombinant proteins more quickly than is possible using traditional protein expression methods, because there is no need to transform, transfect, or culture cells products expressed in CHO expression systems. Since the ExpiCHO kit components have been optimized for high-yield expression in CHO cells, some differences in supernatant clarification and protein purification are to be expected compared to existing transient expression Chinese hamster ovary (CHO) cells are widely used in biopharmaceuticals because of their high-density suspension culture, high safety, and high similarity between expressed exogenous proteins and natural proteins. Kit Type CHO HCP ELISA KIT Complete kit for the determination of Chinese Hamster Overy (CHO) host cell protein contamination bulk products expressed in CHO expression systems. 1. 2. Subculture cells. The provided 96-well plate has been pre-coated with Rabbit anti-CHO HCP antibodies to capture CHO proteins from biological Advancing CHO Protein Production and Engineering CHO cell recombinant expression. The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line, a chemically-defined animal origin-free, serum-free, and protein-free culture medium, an optimized culture feed, and a high-efficiency transfection reagent that work in concert to provide protein titers 3x higher than the Gibco Expi293 Expression System and 160x higher than the Gibco FectoPRO is a transfection reagent specifically optimized for transient protein and antibody expression in CHO and HEK293 cells. hence making this kit an affordable and economical solution for Bioproduction processes. The Another way for regulating gene expression in CHO cells is using the type VI CRISPR system, which includes several effectors such as Cas13a, Cas13b and Cas13d (Abudayyeh et al. The ExpiFectamine CHO Transfection Reagent provides high transfection efficiency of high-density cultures, superior to any other transfection reagent. The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. A11000-01) Product use Learn more about our large selection of high performing Gene Expression Microarray Kits & Reagents for your experiments. They also have the additional advantage of supporting cryopreser - Gxpress CHO Transfection & Medium Kit for CHO Cells “Gene Expression System” Description Gxpress CHO Transfection & Medium Kit has been developed for the gene expression system of Chinese hamster ovary (CHO) cells, such as CHO-K1 or CHO-S cells, and the high production of recombinant proteins or the transfection assay in serum-free culture. The kit provides:GIBCO CD Using components from a commercially available kit, AMPS can purify plasmid preparations from twenty 96-deep-well plates of bridges high-throughput E. The cells have been adapted to CD CHO Medium for serum free suspension FreeStyle™ MAX CHO Expression System USER GUIDE Pub. With ExpiCHO Expression Medium you can: • Grow suspension CHO cultures to densities of over 2 x 10 7 cells per mL, with >95% cell viability The present invention is within the field of industrial protein production. The human cytomegalovirus major immediate early gene (CMV) promoter is currently the most preferred promoter for recombinant therapeutic proteins (RTPs) production in CHO cells. A2910001). 0 ExpiCHO-S™ Cells ExpiCHO™ Expression Medium Description The ExpiCHO-S™ cell line is a clonal derivative of the CHO-S cell line that has been selected for high protein expression. The ExpiCHO Expression System enables: Milligram to multigram yields of recombinant protein per liter of culture in 5–14 days post-transfection; Robust and reproducible transfection results, for greater confidence in results and ability to use CHO cells from research to discovery; Transfection efficiency of > 90% for high-density suspension CHO cell culture, with matching transfection Benefit from access to our proven and scalable CHO based gene expression system to create a diverse range of biologic drugs. The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line, a chemically-defined animal origin-free, serum-free, and protein-free culture medium, an optimized culture feed, and a high-efficiency transfection reagent that work in concert to provide protein titers 3x higher than the Gibco Expi293 Expression System and 160x higher than the Gibco The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. Optimal antibody expression was observed at 1:1 ratio of heavy and light chain in ExpiCHO system. The ultrahigh yields of the ExpiCHO Expression System (up to 2 Overview, continued OptiPRO™ SFM OptiPRO™ Serum Free Medium is included with the FreeStyle™ MAX CHO Expression System to facilitate optimal formation of DNA-lipid complexes. Online exclusive. 8 1. The CHOgro™ Expression System (MIR 6260) is supplied in the following format. These The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line, a chemically-defined animal origin-free, serum-free, and protein-free culture medium, an optimized culture feed, and a high-efficiency transfection reagent that work in concert to provide protein titers 3x higher than the Gibco Expi293 Expression System and 160x higher than the Gibco Total RNAs were extracted from 5 × 10 6 cells using the Gentra Puregene Cell Kit (Qiagen, Hilden, Germany) and the RNAqueous-4PCR kit Different strategies have been used to increase the productivity and quality of protein in CHO expression systems [7, 10, 13, 16]. ④ External tests show that RapidTrans CHO Kit significantly increased protein yields than global This poster presents data and protocol leading to remarkable protein and antibody yields in different transient CHO and HEK-293 expression systems. This poster presents data and protocol leading to remarkable protein and antibody yields in different transient CHO and HEK-293 expression systems. --(BUSINESS WIRE)--#bioproduction--Celltheon today announced the commercial launch of its CHO-K1 based OmniCHO™ Transient Expression Kit, a breakthrough technology poised to transform preclinical drug development. Commun. The provided 96-well plate has been pre-coated with Rabbit anti-CHO HCP antibodies to capture CHO proteins from biological Used to measure residual CHO host cell DNA, this kit uses a 2 ml microfuge tube format and includes our proprietary extraction procedure, which does not use iodine or glycogen. More specifically, the invention pertains to a combination of (i) a DNA vector suitable for production of a recombinant protein, wherein said The kit is for use in determining the presence of host cell DNA impurities (to 1 part per billion) in products manufactured by recombinant expression in CHO cell lines. Expression vectors are one of key elements that influence recombinant ExpiCHO-S™ Cells and ExpiCHO™ Expression Medium Catalog Numbers A29127, A29132, A29100-01, A29100-02, A29100-03, and A29100-04 Pub. 65. DOWNLOADS • FreeStyle™ CHO-S Cells (suspension Chinese Hamster Ovary cells, Cat. as HEK for transient expression before moving to a Chinese hamster ovary (CHO) host for stable expression. Please refer to the manual for additional storage information. --(BUSINESS WIRE)--Celltheon today announced the commercial launch of its CHO-K1 based OmniCHO™ Transient Expression Kit, a breakthrough technology poised to transform The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. The superior co-precipitating reagents do not interfere with qPCR and ensure that pellets do not become dislodged during extraction. Transfect cells and generate protein or virus. This medium is GMP-manufactured, GenScript provides an advanced CHO-HD platform to solve the problem of low yield in HEK 293 cells or other expression system. The ExpiCHO Expression System enables: Milligram to multigram yields of recombinant protein per liter of culture in 5–14 days post-transfection; Robust and reproducible transfection results, for greater confidence in results and ability to use CHO cells from research to discovery; Transfection efficiency of > 90% for high-density suspension CHO cell culture, with matching transfection by expression in the CHO cell line. Another way for regulating gene expression in CHO cells is using the type VI CRISPR system, which includes several effectors such as Cas13a, Cas13b and Cas13d (Abudayyeh et al. The kit does not contain the PCR master mix. Expression levels greater than 30-fold higher are obtained using the ExpiCHO kit as directed versus substitution of expression vector of choice using the ExpiFectamine CHO Transfection Kit and cultured in ExpiCHO Expression Medium (Figure 2). Hundreds of thousands of CHO Host Cell Protein ELISA Kit are in stock. This lead clone was studied in duplicate in a bench-scale bioreactor system with a 2 L working volume using Gibco ™ Dynamis AGT Medium with Effi cientFeed™ Fig. A) was used as the expression vector. Cell counts MIR 6260 1 Kit CHOgro® Expression Medium Chemically defined, hydrolysate-free, animal origin free growth medium supporting transient transfection and AlphaLISA CHO HCP kits are designed to quantitatively measure CHO HCP contaminants in routine bioprocess operations using CHO expression systems, from the very crude harvest material to the final product, in a highly sensitive, quantitative, reproducible, and user-friendly mode (Table 1). The FreeStyle MAX CHO Expression System is a breakthrough technology for the rapid, high-yield production of post-translationally modified mammalian protein in less than one week. 2 Overview, continued OptiPRO™ SFM OptiPRO™ Serum Free Medium is included with the FreeStyle™ MAX CHO Expression System to facilitate optimal formation of DNA-lipid complexes. AlphaLISA CHO HCP kits are designed to quantitatively measure CHO HCP contaminants in routine bioprocess operations using CHO expression systems, from the very crude harvest material to the final product, in a highly sensitive, quantitative, reproducible, and user-friendly mode (Table 1). a representative human igG1 was expressed in CHo suspension cells using multiple transfection methods including: Trans it-Pro™ transfection Kit, 25-kda linear Pei or FreeStyle™ Max transfection Kit and FreeStyle Max with CHo-S cells adapted to FreeStyle CHo medium (Figure 2, blue bars). As market demand continues to rise for more potent and effective therapeutics, drug developers are identifying novel disease targets and biological mechanisms for new medicines. To achieve the highest levels of transient protein expression, the medium is matched to a culture feed, ExpiCHO Feed, also included in the system kit and transfection kits. cantonensis-positive sera in the 2D FreeStyle™ MAX CHO Expression System USER GUIDE Pub. Magic™ Cell-free Protein Expression in Human Hela Cell Lysate Kit is an optimized kit contains a T7 promoter and an EMCV internal ribosome entry site (IRES) to facilitate high levels of in vitro protein expression. OptiPRO™ SFM has an ultra-low protein concentration of 7. Shuting Xu, gave a quick review of the common protein expression systems and introduced the history of CHO cell line in protein expression. CHO cells are robust cells used for the industrial production of a variety of recombinant proteins requiring complex post-translational modifications and correct FreeStyle™ MAX CHO Expression System USER GUIDE Pub. , The CRISPR tool kit for genome editing and beyond. Protocol Outline A. OptiPRO™ SFM The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. It is designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The Gibco™ Freedom™ ExpiCHO-S™ Kit is an easy-to-use solution for cloning and expressing recombinant proteins in suspension cultures derived from Chinese hamster ovary (CHO) cells. Following The kit includes components for transfection, expression, clone creation, and stable cell line selection. Data from sequencing show To demonstrate biotherapeutic relevant protein production a representative human IgG1 was expressed in CHO suspension cells using multiple transfection methods including: Trans ITPRO™ Transfection Kit, 25-kda linear PEI or FreeStyle™ Max Transfection Reagent. B. OptiPRO™ SFM The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. The banked clones can be directly thawed into ExpiCHO SPM for subsequent procedures. FreeStyle™ MAX CHO Expression System Kit Characteristics ∤ Expression system based on CHO-S™ Cells for best compatibility with The RapidTrans CHO Kit supports efficient expression of antigens and antibodies, meeting various application needs. FectoCHO™ Expression system is a complete and easy solution It outperforms all commercially available CHO expression systems, in plug-and-play system thus easy-to-use. ExpiCHO Expression Medium: • Supports growth of suspension CHO cultures to densities of over 2 x 10 7 cells per mL, with >95% cell viability Description. + The system keeps the production of stress-related metabolites such as lactate and ammonia at an absolute minimum during the entire procedure of your fed-batch culture. And then she talked about how to accelerate the research of protein and antibody. 2 mL x 2) *CHO Complexing Solution 80 mL (40 mL x 2) CHO HCP ELISA kit (Host cell protein) (ab240996) is designed to quantitatively measure host cell protein (HCP) contamination in bulk products expressed in Chinese Hamster Ovary (CHO) expression systems. A13696-01), co-developed by Thermo Fisher Scientific Corporation and ProBioGen AG, is designed for easy cloning and expression of recombinant proteins in The FreeStyleTM MAX CHO Expression System facilitates large-scale transient transfection of Chinese Hamster Ovary (CHO) cells, in a deined, serum-free ∤ medium, for expression of The Gibco™ Freedom™ CHO-S™ Kit includes components for transfection, expression, clone creation, and stable cell line selection, all in a conveniently packaged kit. CHO cells are robust cells used for the industrial production of a variety of recombinant proteins requiring complex post-translational modifications and correct This kit is used to check for the presence or absence as well as the amount of CHO host cell protein in the pharmaceutical products produced in this cell, such as recombinant drugs and vaccines. Triplicate flasks of FreeStyle™ CHO-S cells adapted to CHOgro® Expression Medium were transiently transfected with the TransIT-PRO® Transfection Reagent (Mirus Bio) at a 1:1 reagent-to-DNA ratio (vol:wt) and 1 µg plasmid DNA per milliliter of culture at 4 x 10 6 cells/ml in 125 ml Optimum Growth flasks (25 ml/flask, Thomson Instrument The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. Due to The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. A29133) for scalable transient protein production. Note: ™The ExpiFectamine CHO Reagent, ExpiCHO ™ Feed, and ExpiFectamine ™ CHO Enhancer are components of the ExpiFectamine ™ CHO Transfection Kits. R800-07) The CHO cells were suspended in PBS at a density of 1 × 10 7 cells/mL, and 4 × 10 6 cells per cuvette were transfected with both 10 μg of donor vector and 5–25 μg of transposase expression Chinese hamster ovary (CHO) cells comprise a variety of lineages including CHO-DXB11, CHO-K1, CHO-DG44, and CHO-S. CHO HCP ELISA kit (Host cell protein) (ab240996) is designed to quantitatively measure host cell protein (HCP) contamination in bulk products expressed in Chinese Hamster Ovary (CHO) expression systems. Application: Colorimetric detection, ELISA. Plasmid DNA encoding EGFP was delivered by transient transfection with the TransIT-PRO® Transfection Reagent (1:1) (reagent:DNA ratio, volume:weight) using 1 µg plasmid DNA per milliliter of culture and cell a density of 2 x 10 6 cells/ml in the CHOgro® UNION CITY, Calif. However, the level of exogenous protein expression decreases with increasing culture time; this phenomenon occurs due to the recombination of CASE STUDY Freedom CHO-S Kit Freedom CHO-S Kit for stable expression of recombinant proteins in GMP production of 25. The present invention is within the field of industrial protein production. KIT INCLUDES Coated 96-Well Strip Plate CHO Protein Standard 5x Dilution Buffer 10x PBS-T Reporting Antibody Streptavidin-HRP Conjugate Chinese hamster ovary (CHO) cells are common mammalian cell lines for expressing recombinant proteins, yet the expression level of recombinant proteins is still hindered. 0. The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line and an optimized medium and transfection kit that synergistically act to provide titers as much as 160x higher than the Gibco FreeStyle MAX CHO Expression System and 4x higher than the Gibco Expi293 Expression System. The provided 96-well plate has been pre-coated with Rabbit anti-CHO HCP antibodies to capture CHO proteins from biological New CHO system offers ten times higher yields, optimised plasmid stability and smart protocols with less hands-on time. g. Kit, co-developed with ProBioGen AG, is an easy-to-use, beginning-to-end product, for cloning and expression of recombinant proteins in Chinese Hamster Ovary (CHO)-derived suspension culture. CHO HCP ELISA Kit, 3G Cygnus Technologies F550-1 The store will not work correctly in the case when cookies are disabled. CHO cell recombinant expression involves using Chinese Hamster Ovary cells to produce therapeutic proteins through genetic modification, enabling complex post-translational modifications and high-quality protein production. The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line, a chemically-defined animal origin-free, serum-free, and protein-free culture medium, an optimized culture feed, and a high-efficiency transfection reagent that work in concert to provide protein titers 3x higher than the Gibco Expi293 Expression System and 160x higher than the Gibco To achieve the highest levels of transient protein expression, the medium is matched to a culture feed, ExpiCHO Feed, also included in the system kit and transfection kits. With ExpiCHO Expression Medium you can: • Grow suspension CHO cultures to densities of over 2 x 10 7 cells per mL, with >95% cell viability FreeStyle™ CHO-S Cells: Content And Storage: Store FreeStyle cells in liquid nitrogen. A29133) and enables transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. FreeStyle™ CHO-S Cells, Life Technologies® Cat. 12651-014) • DG44 Cells (DHFR – suspension CHO cells) in CD DG44 Medium with 8 mM L-glutamine and 18 mL/L 10% Pluronic™ F-68 (Cat. cspb xpb wvmij azhtz npwusi vuxu drltlo irgik wrowoy gqzha